Enhancement of growth of a mouse macrophage cell line by a tumor promoter and granulocyte-macrophage colony-stimulating factor.
نویسندگان
چکیده
The macrophage-like cell line Mm-i consists of spontane ously differentiated cells of a clonal line of mouse myeloid leukemia cells (Ml). Mm-i cells were seeded in soft agar on a harder agar base in the presence or absence of ascitic fluid from rats bearing hepatoma AH-i 30. Although a few colonies developed in the absence of ascitic fluid, the number of cob nies was significantly increased by addition of the ascitic fluid. Further addition of 12-O-tetradecanoylphorbol-i 3-acetate (TPA) synergistically enhanced formation of colonies of Mm-i cells in the presence of the ascitic fluid, although TPA alone had no effect. In the presence of 2.5 or 5% ascitic fluid, TPA (3.2 x 1O-@M) caused a 1.5to 3-fold increase in the number of colonies over the number without TPA. A positive correlation was found between the tumor-promot ing activities of several plant diterpenes and their enhancing effects on growth of Mm-i cells in semisolid agar in the pres ence of ascitic fluid from rats bearing hepatoma AH-i30. Ascitic fluid contains granubocyte-macrophage cobony-stimu batingfactor (GM-CSF). Conditioned media containing GM-CSF from embryo cells, peritoneal macrophages, and Yoshida sar coma cells, or highly purified GM-CSF's from human urine and mouse L-cells also acted synergistically with TPA in increasing the number of colonies of Mm-i cells. On the other hand, neither epidermal growth factor nor fibroblast growth factor with or without TPA stimulated colony formation of Mm-i cells. These results indicate that colony formation of Mm-i cells is stimulated as a result of the specific interaction of the tumor promoter with growth factor(s), possibly GM-CSF.
منابع مشابه
Expression of a Chimeric Protein Containing the Catalytic Domain of Shiga-Like Toxin and Human Granulocyte Macrophage Colony-Stimulating Factor (hGM-CSF) in Escherichia coli and Its Recognition by Reciprocal Antibodies
Fusion of two genes at DNA level produces a single protein, known as a chimeric protein. Immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. Bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. In this investigation, the catalytic domain of Shiga-like toxin (A1) was fused to human granulocyte macrophage ...
متن کاملImprovement in Development and Quality of 8 Cell Mouse Embryos in Presence of Granulocyte Macrophage-Colony Stimulating Factor
Purpose: Granulose Macrophage-Colony Stimulating Factor (GM-CSF) is a lympho- heamatopoietic actor, secreted in the reproductive system. Murine pre- implantation embryos express GM-CSF receptors. In his study, the capacity of eight cell mouse embryos was studied in the presence and absence of GM-CSF. Materials and Methods: Female NMRI mice were super ovulated using Pregnant Mare Serum gonadotr...
متن کاملThe Expression of Human Granulocyte Macrophage Colony Stimulating Factor by Heat-Induction in Escherichia coli
A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...
متن کاملInduction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)
Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...
متن کاملA Functional Polymorphism of the Granulocyte Macrophage Colony Stimulating Factor is not Associated with the Outcome of HTLV-I Infection
Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Cancer research
دوره 40 2 شماره
صفحات -
تاریخ انتشار 1980